In Depth

The PeakTrace 6 fuse trace at base and fuse basecall at base options allow the combination of 2 basecallers in one basecall to gain the best from both.…

The skip short traces option is a useful option in PeakTrace to skip over the reads from short DNA fragments. As the improvement in basecall quality for shorter traces…

An unfortunate side effect of a basecaller’s ability to process peaks is that the processing can suppress or raise the secondary peak signal and with it alter the…

In most scenarios the usage of PeakTrace will lead to increasing the number of high quality bases in the basecall. There are however a few factors that can  limit PeakTrace's…

One of the most commonly misunderstood options of the PeakTrace™ Basecaller is stealth mode. The specification of the .phd.1 and .scf file requires that the basecaller and trace processor used…

A new feature introduced in Auto PeakTrace 6™ is input overwrite. Under normal circumstances, attempting to set the input and output folder will result in an error when you process the…

One of the more commonly misunderstood settings within PeakTrace™ is the force processing option. This option will allow you to force PeakTrace basecalling of files that would normally be…

The q average trim option allows you to select a quality score average clipping threshold and window size, so that traces are clipped at their 5′ and 3′ ends when the average…

Auto PeakTrace™ 6 (Windows version only) offers support for processing files located inside folders containing unicode characters (non-English), plus the ability to output unicode logfiles. This allows the logfile…

The skip short traces option allows PeakTrace 6™ to skip over traces that have less than a certain number of bases and which may not be improved by PeakTrace. This setting is…