In Depth – Page 3

1 Dec 2016
In Depth: True Profile
One of the more commonly misunderstood settings within PeakTrace™ is true profile. By default PeakTrace uses a flat profile which normalizes the processed peaks to approximately the same height irrespective of the true signal intensity in…
In depth
21 Nov 2016
In Depth: Noise Raw Data
A question we occasionally get asked is how to prevent users from accidentally reprocessing traces if they open them in Sequence Analysis. Since PeakTrace does not change the raw…
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9 Aug 2016
In Depth: Use Edited Basecalls
One of the new features introduced in PeakTrace 6.10 was the use edited basecalls setting. This option allows the retention of manual basecalling changes to the sequence when the fuse trace…
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15 Jul 2016
In Depth: Secondary Noise Peaks
One of the new features introduced in PeakTrace 6.40 was secondary noise peaks. The setting is unusual in that it actually makes the traces appear to be of lower…
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14 Jul 2016
In Depth: Set ABI Limits
One of the new features introduced in PeakTrace 6.40 was Set ABI Limits. This option changes the PeakTrace trimming functions from hard trims (i.e. actual removal of the low quality regions) to the…
In depthNews
11 May 2016
In Depth: Extra Baseline
One of the new features of PeakTrace 6.30 is the extra baseline setting. This setting further improves the appearance of trace files by removing non-base peaks such as dye-blobs…
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28 Apr 2016
In Depth: QualTrace III Trace Reports
The QualTrace III module in PeakTrace 6 can generate text based Trace Report files on each trace file basecalled by PeakTrace. These files can be supplied to final…
How-tosIn depth
12 Apr 2016
In Depth: Mixed Basecalling and Simple Mixed Basecalling
One of the major internal changes within Auto PeakTrace 6 is an improvement to how mixed basecalling (detection of polymorphic sites) is performed. PeakTrace 5 used a similar…
How-tosIn depth
31 Mar 2016
In Depth: Picket Fence Basecalling
One of the new features introduced in PeakTrace 6.10 is the “picket fence” basecalling option. This option is normal PeakTrace basecalling with an extreme peak normalization applied that makes all…
How-tosIn depth
18 Mar 2016
In Depth: PCR Trim Base
One of the new features of PeakTrace 6.20 is user adjustment of the PCR trim base. In previous releases of PeakTrace the PCR trim base was set automatically using…
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