In Depth – Page 4

21 Dec 2015
In Depth: Trim 3-Prime End Only
One of the most simple of the new features within PeakTrace 6 is the trim 3’ end only setting. This option allows you to use trace trimming to remove…
In depth
9 Dec 2015
In Depth: Extra Smoothing Part II
In Part I of this series we looked at how to limit the sharpness of the trace peaks from PeakTrace by using the no peak resolution setting. While this is…
In depth
8 Dec 2015
In Depth: Extra Smoothing Part I
An interesting concern we get from customers about the PeakTrace Basecaller™ is that the peaks are "too sharp" at the end of the trace. This has always puzzled us because…
In depth
1 Dec 2015
In Depth: Extra Normalization
A common problem with Sanger DNA sequencing is a rapid falloff in peak signal strength - what is known colloquially as a “ski-slope” trace. This problem can often be difficult to…
In depth
30 Nov 2015
In Depth: Automatic Bubble Spike Removal
One production problem that can occur with Sanger DNA sequencing is sharp spikes in the raw data channel. These spikes can appear anywhere in the raw channel, are…
In depth
24 Nov 2015
In Depth: Clean Baseline
The clean baseline setting within the PeakTrace Basecaller™ is a cosmetic option that can be used to improve the appearance of trace files. While in an ideal world…
In depth