General information
The Dana-Farber/Harvard Cancer Center (DF/HCC) DNA sequencing core facility offers large and small-scale DNA sequencing to members of the DF/HCC community as well as to external users.
Sequencing instruments used
Applied Biosystems 3730xl
Prices
Single sample
Full service (external customers) | $8.00
Full service (Dana-Fraber/Harvard University customers)
| $6.00
Volume discounts
48+ samples (external customers) | $6.00
48+ samples (Dana-Fraber/Harvard University customers) | $4.00
Other information
Free testing of large project templates and extra discount pricing for large
orders. Free DNA mini-preps for large orders.
Sample requirements
Plasmids
100 ng/µl | 12 µl minimum
PCR products
5 ng/µl per 100 bp | 12 µl minimum
Primers
1.2 pmol/µl | 12 µl minimum
Common universal primers are provided free.
Sample turn around time
24 hours
International orders
Yes
GLP DNA sequencing
No
Other DNA sequencing related services offered
- Plasmid minipreps for glycerol stocks
- Large scale DNA sequencing
Contact Information
HIP/DNA Resource Core
c/o Broad Institute
320 Charles St.
Cambridge, MA 02141
USA
ph. (617) 324-0823
email. jason_kramer@hms.harvard.edu
Comments
Excellent single reaction prices especially for internal customers. Also offer a no charge re-run of failed sequencing reactions (i.e. same templates and primers) to test for problems originating at the sequencing facility.
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