General Information
The facility is located on the campus of the University of Manchester and provides sequencing services to the entire campus and beyond. Clients include research groups as well as private companies.
DNA Sequencing Instruments Used
- ABI 3730
- Roche 454 GS Junior
DNA Sequencing Prices
No information
DNA Template Requirements
The sequencing facility offers two levels of Sanger sequencing:
Pre-mixes
The sample has to contain a mix of primer and template to be sequenced and processed at the facility
Load-only samples
Samples have to be re-sequenced, precipitated to a dry pellet and delivered to the facility to be loaded onto the sequencer.
Sequencing turn-around time
No Information
International orders
No information
GLP DNA sequencing
No information
Other DNA sequencing related services offered
- Metagenomic analyses
- Next-generation sequencing projects
- Human cell line authentication (Fragment analysis)
- Genotyping services (eg microsatellite, TRFLP analysis)
- Mycoplasma testing (PCR-based)
Contact Information
The University of Manchester
Oxford Rd
Manchester
M13 9PL
United Kingdom
Paul Fullwood
Tel: +44 (0)161 275 4867
Email: dnasequencing@manchester.ac.uk
Website: https://www.bmh.manchester.ac.uk/research/facilities/dna-sequencing/
Comments
The facility claims that Sanger sequencing and Roche 454 pyrosequencing can provide read lengths of >1000bp. Sequencing facility staff provide troubleshooting, sequencing advice, and assistance with sequence data analysis.