The facility is located on the campus of the University of Manchester and provides sequencing services to the entire campus and beyond. Clients include research groups as well as private companies.
DNA Sequencing Instruments Used
- ABI 3730
- Roche 454 GS Junior
DNA Sequencing Prices
DNA Template Requirements
The sequencing facility offers two levels of Sanger sequencing:
The sample has to contain a mix of primer and template to be sequenced and processed at the facility
Samples have to be re-sequenced, precipitated to a dry pellet and delivered to the facility to be loaded onto the sequencer.
Sequencing turn-around time
GLP DNA sequencing
Other DNA sequencing related services offered
- Metagenomic analyses
- Next-generation sequencing projects
- Human cell line authentication (Fragment analysis)
- Genotyping services (eg microsatellite, TRFLP analysis)
- Mycoplasma testing (PCR-based)
The University of Manchester
Tel: +44 (0)161 275 4867
The facility claims that Sanger sequencing and Roche 454 pyrosequencing can provide read lengths of >1000bp. Sequencing facility staff provide troubleshooting, sequencing advice, and assistance with sequence data analysis.