General Information
The core facility for DNA sequencing established in 1992 was merged in 2008 to form the new core facility KIGene – genetic analysis at CMM located at the Department of Molecular Medicine and Surgery, the Neurogenetics Unit, CMM L8:00, Karolinska University Hospital at Solna.
DNA Sequencing Instruments Used
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Applied Biosystems 3730
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Applied Biosystems 3130xl
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Life Tech Ion Proton
DNA Sequencing Prices
Pre-made reactions
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Analysis of DNA sequence reaction | 60 SEK/sample
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96 sequence reactions in 96-well plate (no printouts) | 30 SEK/sample
Full service
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DNA sequence reaction, clean-up, analysis | 160 SEK/sample
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48 DNA sequence reactions, clean-up, analysis (no printouts)| 80 SEK/sample
DNA Template Requirements
Plasmids
No information
PCR Products
No information
Primers
No information
Other information
Full-service: The user provides starting materials- as blood, saliva, biopsies, DNA, oligonucleotides and the facility performs the analysis.
Sequencing turn-around time
12-24 hours
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
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Next Generation Sequencing
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Genotyping
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Fragment Analysis
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Quantitative PCR
Contact Information
KIGene
MMK, Neurogenetics Unit, CMM L8:00
Karolinska University Hospital at Solna
171 76 Stockholm
Sweden
Phone: 08-517 725 42
Email: kigene@mmk.ki.se
Website: http://ki.se/en/research/kigene-genetic-analysis-at-cmm
Comments
Very fast turnaround time provides results on the same day if samples are submitted before 10 am and the pre-made samples before 1pm. The facility has experiences of two decades and provides start up theoretical and technical assistance. Generate high quality sequencing up to 700 bases. The facility stores all data from pre-made and full-service sequence samples.