General Information
Founded in 1995, the MGH DNA Core, now known as the Center for Computational and Integrative Biology (CCIB) DNA core is a well-established major research core laboratory within the Center for Computational and Integrative Biology of Massachusetts General Hospital Boston. The majority of clients at the core are investigators from Massachusetts General Hospital and the greater Partners research community. They also serve researchers from external academic and medical centers as well as investigators from industry throughout the United States and across the world.
DNA Sequencing Instruments Used
- 3 x ABI 3730xl
DNA Sequencing Prices
Individual tube
Partners HealthCare Members | $3.49
External Academic and Medical Centers | $5.03
For-Profit Organizations | $5.55
Bulk order (95 samples)
Partners HealthCare Members | $2.16
External Academic and Medical Centers | $3.11
For-Profit Organizations | $3.43
DNA Template Requirements
Cosmids
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- 400ng/ µl
Plasmids
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- < 10kb | 100 -200 ng/µl
- > 10kb | 200 ng/µl
PCR Product
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- < 0.5 kb | 10-20 ng/µl
- 0.5kb – 1kb | 20-50 ng/µl
- > 1kb | 50-100 ng/µl
Primers
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- 1.5-3.0 µM
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Sequencing Turn Around Times
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- 24 hours
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Other DNA sequencing related services offered
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- Long read Sanger sequencing services
- Difficult DNA template Sanger sequencing services
- Complete plasmid sequencing
- CRISPR sequencing
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Contact Information
CCIB DNA Core Facility at MGH
38 Sidney Street (Suite 100)
Cambridge, MA 02139
Ph: 617-726-5982
Email: DNACORE@CCIB.MGH.HARVARD.EDU
Website: https://dnacore.mgh.harvard.edu/new-cgi-bin/site/pages/index.jsp
Comments:
The sequencing experts at the CCIB Core inspect each individual chromatogram before posting the results on their website, meticulous practices like these make CCIB a competitive service in Massachusetts.
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