General Information
The DNA Sequencing & Genotyping Center (SGC) is located in the Delaware Biotechnology Institute. The Delaware Biotechnology Institute is an interdisciplinary research unit at the University of Delaware.
DNA Sequencing Instruments Used
- ABI Prism 3130XL
- Illumina HiSeq 2500
- Pacific Biosciences RSII Single-Molecule Sequencer
- Illumina MiSeq
- ONT MinIon
DNA Sequencing Prices
University of Delaware Investigators
- Less than 96 sequences | $7.50 per sequence
- Full 96-well plate | $384 per plate or $4 per sequence
- Fragment Analysis | $3 per sample
External Investigators (outside UD)
- Less than 96 sequences | $10 per sequence
- Full 96-well plate | $480 per plate or $5 per sequence
- Fragment Analysis | $4 per sample
DNA Template Requirements
- Single-stranded plasmid | 15-45 ng/µl
- Double-stranded plasmid | 50-75 ng/µl
- BAC, YAC, PAC, cosmid | 400-800 ng/µl
- Bacterial genomic DNA | 1-3 µg/µl
PCR product: (10µl)
- 100-200 bp | 2-4 ng/µl
- 200-500 bp | 5-10 ng/µl
- 500-1000 bp | 10-15 ng/µl
- 1000-2000 bp | 15-30 ng/µl
- >2000 bp | 30-50 ng/µl
Primers
Primers should be re-suspended in 10mM Tris pH 8.0 or sterile H2O at a concentration of 2µM | (2pmol/µl)
Other information
Templates should be re-suspended in 10mM Tris pH 8.0 or sterile H2O. Tris EDTA buffer (TE) is not recommended because EDTA may inhibit subsequent enzymatic reactions.
Sequencing Turnaround Times
Less than 3 working days
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
- Next Generation Sequencing
- Single-molecule sequencing,
- Qualitative and quantitative analysis of nucleic acid
Contact Information
Delaware Biotechnology Institute
Address: Room 246, 15 Innovation Way, Newark, DE 19711 USA
Brewster Kingham
Phone: +1 302 831 0823
Fax: +1 302 831 4841
Email: dnasequence@udel.edu
Website: http://www.udel.edu/dnasequence/Site/Home.html
Comments
The SGC provides assistance with experimental design, user training, sample and data analysis. University of Delaware DNA Sequencing & Genotyping Center is using iLab Solutions. iLab solutions is an online system to streamline the process of service requests, sample submission, and sample tracking.
Return to the main DNA sequencing services review page.