General Information
The Molecular Resource Center (MRC), established in 1985, is one of the Tennessee Higher Education Commission (THEC) Centers of Excellence at the University of Tennessee Health Science Center (UTHSC). The MRC facilitate molecular genetics and biochemical studies at the sub-cellular and systems biology levels.
DNA Sequencing Instruments Used
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Applied Biosystems 3130xl
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Ion Torrent PGM
DNA Sequencing Prices
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UT: $5 /sample
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Non-UT academic: $6/sample
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Non-academic: $7/sample
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Complex/difficult samples academic | $12/sample
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Complex/difficult non-academic | $14/sample
DNA Template Requirements
Plasmids
- 700 ng
Single Stranded DNA
- 10-50 ng
PCR products
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100 to 200 bp | 3-10 ng
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200-500 bp | 10-20 ng
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500 to 1000 bp | 20-40 ng
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1000-2000 bp | 40-50 ng
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>2000 bp | >50 ng
Primers
10 pmol
Other information
A few standard primers are provided by the facility. All samples are submitted through DNAlims website.
Sequencing turn-around time
No information
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
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Next Generation Sequencing
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Library Construction
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Fragment Analysis
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Genotyping
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Micro Array Analysis
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Quantitative PCR
Contact Information
Molecular Resource Center
Cancer Research Building
19 S. Manassas, Suite 341
Memphis, TN 38163
USA
Phone: +1 901 448 2656 | +1 901 523 8990
Email: rraghow@uthsc.edu
Website: http://www.uthsc.edu/research/molecular_resource/
Comments
MRC provide services as well as technical assistance on cost basis. The MRC’s services include sequencing at both the single fragment and whole genome (next generation) levels along with affymetrix microarrays. The center provides accurate sequence up to ~700 bp or greater for Sanger sequencing. This could be much higher if they used the best software available. Prior arrangements must be made with the center for data analysis.