The N Base Trim option allows users to trim the 3' or 5' end of a trace based on the number of N base calls in a selected window size. For example, if you were to use the values of 5N bases in a window of 20 bases, PeakTrace would use a sliding … [Read more]
How to update the CodeMeter Runtime
All version of Auto PeakTrace above 5.70 require the latest CodeMeter Runtime (6.90 as of September 2019). To update you first need to check which version of the CodeMeter Runtime you have installed on your computer. To do this: 1. Open the … [Read more]
Auto PeakTrace 6.936 & PeakTrace 6.936 Released
We have released Auto PeakTrace 6.936 and PeakTrace 6.936 for Windows & Linux. This is a minor release that squashes a few bugs that have been uncovered over the last couple of months. This update is recommended for all users of the 6.93 series … [Read more]
Auto PeakTrace 6.935 & PeakTrace 6.935 Released
We have released Auto PeakTrace 6.935 and PeakTrace 6.935 for Windows & Linux. This is a minor release that improves the basecalling why large or late air bubble spikes are present in the raw data. This update is optional, but if you have noticed … [Read more]
Auto PeakTrace 6.934 & PeakTrace 6.934 Released
We have released Auto PeakTrace 6.934 and PeakTrace 6.934 for Windows & Linux. This is a small bug fix release that corrects an issue with the simple mixed basecall as well as a rare thread race issue when archive traces and auto start are both … [Read more]
Why use PeakTrace?
A question we get asked frequently is why should a core facility or sequencing service company use the PeakTrace Basecaller™ -- after all the KB Basecaller™ works well and is effectively free since it comes with the DNA … [Read more]
In Depth: Q Count Trim
The q count trim option allows end trimming of the low quality regions of the basecall using a count of q score below a set threshold using a window of variable size. This trim option is a combination of 3 parameters; a quality score threshold, the … [Read more]
In depth look at the new features of PeakTrace 6
PeakTrace 6 (Auto PeakTrace 6, Auto PeakTrace 6 RP and the Linux PeakTrace 6) has a number of very powerful options that may not have been explored by many users of older versions of PeakTrace. We understand that most of our customers are busy … [Read more]
In Depth: Auto Start
Under the default settings, Auto PeakTrace 6 will only start to process trace files after the user has manually click the Run button on the main window. If the auto start option is checked then Auto PeakTrace will automatically begin processing of … [Read more]
In Depth: Archive Traces
A problem that may be encountered when using Auto PeakTrace™, is reprocessing traces that have not been moved from the input folder since PeakTrace was restarted. This can be avoided by using the archive traces options. When selected, … [Read more]
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