General Information
The DNA sequencing core facility at Tufts University supplied DNA sequencing and other related services to researchers at Tufts as well as outside organizations. The core facility was established in 1987 and currently has six staff members.
Sequencing Instruments Used
- Applied Biosystems 3130xl.
- Illumina HiSeq 2500.
- Illumina MiSeq.
DNA Sequencing Prices
Single sample | Plasmids & PCR products
Small plasmids & PCR products | $5.00
BAC, Fosmids & Genomic DNA | $6.00
Sample Requirements
PCR product
10- 150 ng/µl | 10 µl minimum
The facility must be told the size of the PCR product.
Plasmid DNA
40-150 ng/µl | 300 ng total
BACs, Cosmids and Genomic DNA
Contact for details.
High Volume
Samples must be supplied premixed with the sequencing primer in 96 well traces.
Primers
5 pmol/µl | 5µl
Common sequencing primers provided at no cost.
Other details
All samples must be supplied in either water or Tris (no EDTA). Water is preferred.
Sample Turn Around Time
24-48 hours, average of 36 hours
International Orders
Unknown
GLP DNA Sequencing
No
Other DNA sequencing related services
- Oligonucleotide synthesis
- MALDI-TOF Mass Spectrometry
- NextGen DNA sequencing on Illumina platforms, HiSeq 2500 and MiSeq.
Contact Information
Tufts University Core Facility
Tufts Medical School
Dept. of Physiology/ST-808
136 Harrison Ave. Boston, MA 02111
USA
ph. 617-636-2422
fax. 617-636-6737
email. michael.berne@tufts.edu
website. http://www.tucf.org/
Comments
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