General Information
VANTAGE (Vanderbilt Technologies for Advanced Genomics) is the core genomics laboratory for the Vanderbilt University Medical Center and offers a wide range of DNA sequencing and next generation genomic services. Based in Nashville, Tennessee.
DNA Sequencing Instruments Used
ABI 3730xl (96 capillary instrument)
Sanger DNA Sequencing Prices
Full service DNA sequencing
Single tube | $8.25 (VU); $9.90 (academic); $13.20 (commercial)
Full plate | $450.00 (VU); $540.00 (academic); $720.00 (commercial
DNA Template Requirements
Plasmids
100 ng/µl (10µl)
PCR products
< 250 bp | 35ng/µl (10µl)
250 – 500 bp | 35 – 50ng/µl (10µl)
500 – 700 bp | 50 – 75 ng/µl (10µl)
> 700 bp | 100ng/µl (10µl)
Primers
1 µM (10 µl)
Other information
They evaluate the performance of two negative and five positive control samples, processed in randomized positions on each plate and check each trace for signal strength, read quality and length. Reactions are considered to pass if they contain fewer than 10% low quality bases prior to position 600.
Sequencing Turn Around Times
48 hours
International orders
Unknown
GLP DNA sequencing
No
Contact Information
VANTAGE (Vanderbilt Technologies for Advanced Genomics)
T0102 Medical Center North
Vanderbilt University Medical Center
Nashville, TN 37232
USA
website. http://vantage.vanderbilt.edu
phone. (615) 936 3003
Comments
The VANTAGE (Vanderbilt Technologies for Advanced Genomics) offer a very high quality service. The DNA sequencing quality is excellent and they offer PeakTrace processing of there traces. They are also friendly and professional. Highly recommended.
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