General Information
This Sequencing Unit is part of the Institute of Genetics of the Ludwig-Maximilians-University in Munich. The Institute focuses on the molecular mechanisms that regulate organismic interactions between eukaryotic host cells and symbionts, parasites and pathogenic microbes.
DNA Sequencing Instruments Used
Two ABI 3730
DNA Sequencing Prices
No Information
DNA Template Requirements
Plasmids
plasmids | 150-300 ng
cosmids or BACs | 300-1000 ng
PCR products
100-200 bp | 5-20 ng
200-500 bp | 10-40 ng
500-1000 bp | 20-50 ng
1000-2000 bp | 40-100 ng
> 2000 bp | 50-150 ng
Primers
2-10 pmol depending on primer sequence (3.2 pmol is indicated as a good starting point to find the optimal concentration)
Other information
The facility offers full sequencing service (Cycle, clean and run), but also “Clean and run” and “Run” only.
The facility gives a specific protocol on their website for template preparation, cleaning, or cycling conditions respectively.
Sequencing turn-around time
No Information
International orders
Yes
GLP DNA sequencing
No Information
Other DNA sequencing related services offered
- Fragment analysis
Contact Information
Sequencing Service, LMU Biozentrum
Großhaderner Str. 2-4,
82152 Martinsried
Germany
Phone: +49 89-2180-74715
Website: www.genetik.biologie.uni-muenchen.de/service/genomicsserviceunit/index.html
Contact: Dr. Brachmann Andreas – email brachmann@lmu.de
Comments
The sequencing service seems to be targeted mainly at internal clients, but samples can also be sent by mail. For sequencing services, the customer can choose between BigDye v1.1 and BigDye v3.1 sequencing chemistry.
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