General Information
The Bauer Core Facility provides state-of-the-art instrumentation and expertise to scientists at Harvard. Their goal is to advance research efforts in the life sciences that cannot readily be accomplished in a traditional academic laboratory because of a need for expensive instrumentation, scientific or organizational infrastructure, or multidisciplinary expertise.
DNA Sequencing Instruments Used
- ABI 3730xl
DNA Sequencing Prices
Staff Run, per 8 sample strip
- Harvard account user | $18.00
- Purchase order Payment | $23.00
Self run, per half plate
- Harvard account user | $23.00
- Purchase order Payment | $31.00
DNA Template Requirements
template requirements are per 8.0 µL Big Dye Reaction mix, bringing total volume to 20 µL
DNA template
- 15-30 fmoles
Primers
- 3.2pmoles
Other information
The website also contains information on economizing Big Dye reactions by giving requirements for halved or quartered reactions.
Sequencing Turn Around Times
For staff run reactions the data will be available on the server within 17 hours.
International orders
- Not listed
GLP DNA sequencing
- Not listed
Other DNA sequencing related services offered
The Bauer Facility can support massively parallel high throughput sequencing using Illumina HiSeq and NextSeq instruments.
Contact Information
Bauer Core Facility
Harvard University
Northwest Building, room B239
52 Oxford St
Cambridge, MA 02138
Email: claire@cgr.harvard.edu
Ph: 617-384-7913
Website: https://bauercore.fas.harvard.edu/
Comments
The technicians at the Bauer Facility are happy to consult with researchers to find the best technologies available at the Core and provide them with in depth knowledge on completing sequencing projects.
Return to the main DNA sequencing services review page.