General Information
Located on the Cornell Ithaca campus in upstate New York, the Biotechnology Genomics Institute provides a hub for cutting edge scientific facilities, education and training. The Genomics facility supply a range of genomics services from Sanger sequencing to Next Generation Sequencing.
DNA Sequencing Instruments Used
- ABI3730XL
DNA Sequencing Prices
| Service | Internal (Cornell and Cornell Affiliates) | External | External, NYSCAT subsidized (for-profit, New York State Companies ONLY) |
|---|---|---|---|
| Full Service Sequencing, plasmid or PCR product, per sample, template premixed with sequencing primer | $4.25 | $6.80 | $5.44 |
| Special Conditions–dGTP addition, BAC sequencing, added to the single sample sequencing cost, per reaction | $5.00 | $8.00 | $6.40 |
| Full Service Sequencing, plasmid or PCR product, per 96-well plate, templates premixed with sequencing primer, 94 samples | $300.00 | $480.00 | $384.00 |
| Ready-to-Load Sequencing, per 96-well plate | $42.50 | $68.00 | $54.40 |
| Fragment analysis, ready-to-load, per 96-well plate | $42.50 | $68.00 | $54.40 |
- Price per sample
- Large batch of samples (71 or more) can be summitted in plate and run at a reduced cost (plate pricing)
- Universal primers are supplied with additional fee: $1 per sample tube for internal customers and $1.60 per sample tube for external customers
- Re-order of data results: $5 internal and $8 external fee per order
DNA Template Requirements
Plasmids
- 1µg/µl | volume of 18µl (with adding primer) or 10µl (without adding primer)
PCR products
- DNA = {base pairs}/ 5 | volume of 18µl (with adding primer) or 10µl (without adding primer)
- Maximum PCR product concentration is 100ng/µl
Phage Lambda, Cosmid and BAC
- Contact staff
Primers
- 25pmole of primer
- Common universal primers are provided with fee.
Sequencing Service List
- Full DNA Sequencing
- Ready-to-load DNA Sequencing
- Fragment analysis
Sequencing turn-around time
1-2 days from the arrival time of samples.
International orders
Not mentioned
Other DNA sequencing related services offered
- Next Generation Sequencing
- Full Service Genomics Projects
- Nucleic Acid Extraction and Sample Preparation
- Library Construction for NGS
- Bioinformatic
- Protocol Development
- Consultation
Contact Information
Room 147 – Biotechnology Building
526 Campus Road
Cornell University
Ithaca NY 14853
USA
Comments
Cornell appear to provide a DNA sequencing service with fast turn-around times, but high pricing. Universal primers also have a fee, which is uncommon.
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