General Information
The Department of Biological Sciences DNA Core facility at Florida International University (FIU) is fully equipped for DNA sequencing, fragment analysis, and data analysis.
DNA Sequencing Instruments Used
- Applied Biosystems 3100
DNA Sequencing Prices
- Load-only | $4.00 /reaction
- Full service | $15.00 /reaction
- Template quantitation | $2.00/ reaction
- Fragment analysis | $4.00/ reaction
- Cleaning samples | $3.00/ reaction
DNA Template Requirements
Samples must be submitted in 0.2ml PCR tubes. Total volume of 10µl
Single-stranded DNA
- 50-100 ng
Double-stranded DNA (Plasmid)
- 250-500 ng
Cosmid, BAC
- 0.5-1.0 µg
Bacterial genomic DNA
- 2-3 µg
PCR Products
- 100-200 bp | 1-3 ng
- 200-500 bp| 3-10 ng
- 500-1000 bp| 2-20 ng
- 1000-2000 bp| 10-40 ng
- >2000 bp | 40-100 ng
Primers
10µM
Other information
If a sample fails to produce any readable sequence due to a technical error it will be rerun automatically, using the original sample, at no additional charge.
Sequencing turn-around time
2-3 days
International orders
No
GLP DNA sequencing
No
Other DNA sequencing related services offered
- Fragment Analysis
Contact Information
Paul Sharp. Manager
DNA Sequencing Core Facility
Health and Life Sciences Building, Room 332.
11200 S.W. 8th Street
Miami, Florida 33199
USA
Phone: +1 (305)348-0376
Email: dnacore@fiu.edu
Website: http://www2.fiu.edu/~dnacore/index.html
Comments
This service is available only for FIU Faculty and Students. The facility will store the results for 30 days to retrieve your data. Typical read length is 500 to 800 nucleotides. This could be better if they used the best DNA sequencing basecalling software available.
Return to the main DNA sequencing services review page.