General Information
The Genomics Resource provides support for genetic and expression analyses and services in three laboratory facilities – 1) DNA Array, 2) Genetic Analysis, and 3) RNAi. The services include Oligo and cDNA microarray technologies, NextGen and Sanger DNA sequencing, genotyping, quantitative (real-time) PCR, shRNA/miRNA/siRNA screening technologies.
DNA Sequencing Instruments Used
- Applied Biosystems 3730xl
- Illumina HiSeq2500
- Roche 454 GS Junior
DNA Sequencing Prices
Not stated
DNA Template Requirements
Plasmids
- <6 kb | 25-150ng/µl
- 6 to 10 kb | 150-275ng/µl
- >10 kb | 275-500ng/µl
Single-stranded DNA
- 5-25ng/µl
Cosmid or BAC DNA
- 100-500ng/µl
Bacterial genomic DNA
- 500-1500ng/µl
PCR Products
- 100 to 200 bp | 0.25-1.5 ng/µl
- 200 to 500 bp | 0.5-5.0ng/µl
- 500 to 1000 | 1.0-10ng/µl
- 1000 to 2000 | 2.5-25ng/µl
- >2000 | 5-25ng/µl
Primers
- 3 µM
Other information
All DNA templates and primers should be resuspended in water or Tris buffer. M13Reverse, M13Forward, T7, and SP6 available without additional charge.
Sequencing turn-around time
48 hours
International orders
No
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
- Next Generation Sequencing
- Library Preparation
- Fragment Analysis
- Genotyping
- Quantitative PCR
- Micro Array Analysis
Contact Information
Jeff Delrow, Ph.D., Director
The Genomics Resource
Fred Hutchinson Cancer Research Center
Thomas Bldg DE-740
P.O. Box 19024, DE-740
Seattle, WA 98109-1024
USA
Phone: +1 (206) 667-2714
Fax: +1 (206) 667-2825
Email: genomics@fhcrc.org
Website: http://sharedresources.fhcrc.org/core-facilities/genomics
Comments
New customers are strongly encouraged to meet with lab personnel prior to planning a project. Two options, “full-service” and “ready-to-go”. For failure reactions, if the repeat works you will not be charged for the repeat.
Return to the main DNA sequencing services review page.