General Information
The Biopolymers Facility is a non-profit technology service center in the Department of Genetics at Harvard Medical School. Their mission is to provide investigators with access to technology services that will help them speed along their research programs while conserving both time and money by not having to conduct the experiments within their own labs.
DNA Sequencing Instruments Used
- ABI 3730xl
- Illumina HiSeq 2000, 2500
- Illumina MiSeq
DNA Sequencing Prices
(The prices are for Harvard/ Non-Harvard Academic/Commercial respectively)
- 384-Well Standard Bulk Sample Order- $3.96/ $4.55/ $5.15
- 96-Well Standard Bulk Sample Order- $3.96/ $4.55/ $5.15
- Standard Individual Sample -$3.96/ $4.56/ $5.15
DNA Template Requirements
- Microtube: 15µL or 30 µL
- 96 well plate: 15 µL
Plasmid
- 500 ng (15 µL)
- 1000 ng (30 µL)
PCR product
- 50 ng/100 base pairs (15 µL)
- 100 ng/100 base pairs (30 µL)
Single Stand DNA
- 100 ng (15 µL)
- 200 ng (30 µL)
Primer
- 4 pmol (15 µL)
Other Information
If you want the core to have the ability to repeat your reactions in the event of failure without requesting additional sample, please provide 30µl.
Sequencing Turn Around Times
48 hours
International Orders
Yes
GLP DNA sequencing
No information
Other DNA sequencing related services offered
- Library Preparation
- Sequencing by Synthesis
- Exome and targeted sequencing
- Whole transcript sequencing
- Small RNA sequencing
- Mitochondrial sequencing
Contact Information
Harvard Medical School
Department of Genetics
77 Ave. Louis Pasteur
NRB Room 0088
Boston, MA 02115
Phone: +1 617 432 7481 | +1 617 432 7481
Email: dnaseq@genome.med.harvard.edu, nextgen@genome.med.harvard.edu
Website: http://genome.med.harvard.edu/
Comments
The investigators at Harvard Medical School make up the primary client base of the BPF. Through the University’s partnerships serve labs throughout the entire Longwood Medical Area (LMA). While the majority of their users are from the LMA they also serve labs at schools, universities and biotechnology companies from all across the world.
Return to the main DNA sequencing services review page.