General Information
The Hubbard Center for Genome Studies was established in June 2001 to lead the development of genomics research at University of New Hampshire. The HCGS provide technical assistance by constructing BAC and cDNA libraries, providing services in high-throughput DNA sequencing, fragment analysis and analysis of gene expression. It also will maintain expertise in biocomputing and database services to support these activities.
DNA Sequencing Instruments Used
- ABI3130 genetic analyzer
DNA Sequencing Prices
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Ready to Load reaction: $3/sample
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Template/primer provided (sequencing reaction and cleanup): $6/sample
DNA Template Requirements
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Double-stranded (including high copy number plasmids) | 150-300 ng
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Single-stranded | 20-50 ng
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Cosmid, Fosmid, BAC | 500-1000 ng (0.5-1.0 µg)
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Bacterial genomic DNA | 2000-3000 ng (2-3 µg)
PCR products
- (100-200 bp) 1-3 ng
- (200-500 bp) 3-10 ng
- (500-1000 bp) 5-20 ng
- (1000-2000bp) 10-50 ng
- (>2000 bp) 20-50 ng
Primers
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For sequencing of PCR products, single-stranded DNA, and double-stranded DNA- 2µM
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For cosmid, fosmid, and BAC sequencing 5 µM
Other information
Please note on the submission form when you are submitting PCR PRODUCTS (less than 400bp) or if the G+C CONTENT IS GREATER THAN 65%.
Sequencing Turn Around Times
24-48 hours
International orders
No information
GLP DNA sequencing
No information
Other DNA sequencing related services offered
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Fragment analysis
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Gene expression analysis
Contact Information
Hubbard Center for Genome Studies
Gregg Hall, UNH,
Durham, New Hampshire 03824
USA
Email: hcgs.core@unh.edu
Website: http://dnacore.unh.edu/
Comments
HCGS operate on first-come, first-served basis. If you want to discuss protocols or results in person, please feel free to contact the core facility personnel to schedule a time to do so. They are happy to discuss your data and samples with you, but may not have time to do so if you just call or drop by (there are many users compared to the staff)