General Information
The DNA Sequencing Core Facility located within Department of Biochemistry & Molecular Biology of Indiana University School of Medicine offers an automated DNA sequencing service for sequence analysis.
DNA Sequencing Instruments Used
- Applied Biosystems 3100
DNA Sequencing Prices
- $13.00/sample (less than 30 samples submitted at one time)
- $11.00/sample (30 samples & more submitted at one time)
- $7.50/sample (96 samples & more submitted at one time)
DNA Template Requirements
Plasmids
- 1kb – 10kb | 200ng/µl
- 10kb – 20kb | 400ng/µl
PCR Products
- 100 – 500bp | 05 – 20ng/µl
- 500 – 1000bp | 20 – 50ng/µl
Primers
- 3 pmol/µl
The core provides many sequencing primers at no additional cost.
Other information
The mentioned sequencing charges are for ds, ss, and PCR products. For others DNA products please contact sequencing personnel for detail information.
Sequencing turn-around time
24 hours
International orders
No
GLP DNA sequencing
No
Other DNA sequencing related services offered
- Fragment Analysis
Contact Information
Oun Kheav, Director
DNA Sequencing Core Facility
1481 West 10th Street, room D-3013
Indianapolis, Indiana 46202
USA
Phone: +1 317-988-3645
Fax: +1 317-988-2527
Email: okheav@iu.edu
Website: http://biochemistry.iu.edu/resources/dna-sequencing-core-facility/
Comments
The core has capacity to perform about 500 samples per week. DNA samples and primers are stored for two weeks. The core provides DNA sequencing service only for IU investigators.
The Sanger sequencing service was shut down in 2017
Return to the main DNA sequencing services review page.