General Information
The Iowa Institute of Human Genetics (IIHG) is supported by the University of Iowa Carver College of Medicine. IIHG Genomics Division (formerly the DNA Facility) provides resources for functional genomics and recombinant DNA technology.
DNA Sequencing Instruments Used
- Applied Biosystems 3730xl
- Illumina HiSeq & MiSeq
DNA Sequencing Prices
Individual Tube | $6.75/reaction
96 well plate| Division performs chemistry: $3.05*/reaction
96 well plate| User performs chemistry: $0.95/reaction
*Plate submissions will be charged in multiples of 48 reactions.
DNA Template Requirements
Plasmids
- (1.5 to 8 kbp) | 0.5µg
- More than 8 kbp | 1ug
PCR products
- 10 ng/100 bp
Primers
- 6-10 pmoles
Other information
Universal primers are provided by the division upon request.
Sequencing turn-around time
24-48 hours
International orders
Yes
GLP DNA sequencing
Yes
Other DNA sequencing related services offered
- Next Generation Sequencing
- De novo Sequencing
- smRNA discovery and quantification
- RNA Sequencing
- Quantitative PCR
- Oligosynthesis
- Microarray Analysis
Contact Information
IIHG Genomics Division
Director: Kevin Knudtson, PhD
323 Eckstein Medical Research Building (EMRB)
285 Newton Road
Iowa City, IA 52242
USA
Phone: +1 319 335 6736
Fax: +1 319 335 6737
Email: dna-sequencing@uiowa.edu
Website: http://www.medicine.uiowa.edu/humangenetics/
Comments
IIHG offers Sanger sequencing at low cost. The samples are charged in multiples of 48. Members of the IIHG Bioinformatics Division perform a preliminary analysis (e.g., assembly, alignment, etc.) on all runs. The results can be downloaded via a web browser through the website.
Return to the main DNA sequencing services review page.