General Information
Laval University’s Institute f0r Integrative Systems Biology (IBIS) was established in 2009 to meet the understand of the complexity behind biological systems. At IBIS research is organised independently of research domains, specialities and expertise.
DNA Sequencing Instruments Used
ABI3130 (16 capillary)
DNA Sequencing Prices
| Sequencing | Price | Facturation code |
|---|---|---|
| Plasmid | 4.00$ | RXS1 |
| PCR Product | 4.00$ | RXS2 |
| Phage | 4.00$ | RXS3 |
| m13 clone (single-stranded DNA) | 4.00$ | RXS4 |
| BAC, YAC, cosmid | 12.00$ | RXS6 |
| ExoSAP-IT treatment | +1.00$ | RXS7 |
| dGTP chemistry | +0.30$ | RXS8 |
| Special PCR conditions | +1.00$ | RXS9 |
| GC-rich sample | +1.00$ | RXS10 |
| Tube replacement | +1.00$ | RXS11 |
| Electrophoresis (see Notes) | ||
| Migration resuming (per sample) | 3.00$ | MIG2 |
| 16 samples lot migration | 50.00$ | MIG3 |
| Fragments Analysis (see Notes) | ||
| T-RFLP, AFLP, microsatellites | ||
| 16 samples lot migration | 56.00$ | RXS15 |
Notes:
- Prices (in Canadian dollar, $CAD) are, unless otherwise indicated, for 1 reaction/sample, and are subject to modification without notice.
- Clients outside Laval University must add 15% to the price shown for administrative expenses as requested by Laval University.
- 24h Ultra-fast service availability must be confirmed by phone with the manager (418-656-2131 ext 2552).
- Rate for Fragments Analysis are for electrophoresis only. It do not include bioinformatics analysis.
- For items Electrophoresis and Fragments Analysis, prices are given for a minimum of 16-samples lot. If 18 samples are sent, the customer will be charge for 2 lots.
DNA Template Requirements
DNA template
| DNA type | Concentration | Volume reaction | Volume minimal |
|---|---|---|---|
| Lambda clones (phages) | 100 ng/μl | 2 μl | 10 μl |
| Plasmids | 100 ng/μl | 2 μl | 10 μl |
| m13 clones (single-stranded DNA) | 25 ng/μl | 2 μl | 10 μl |
| Purified PCR products | |||
| – fragments of <1 kbp | 2 ng/μl | 2 μl | 10 μl |
| – fragments of 1-2kbp | 5 ng/μl | 2 μl | 10 μl |
| – fragments of >2kbp | 10 ng/μl | 2 μl | 10 μl |
| Non-purified PCR products | >10 ng/μl | 5 μl* | |
*5μl exactly because they will add an exact extra volume. They can do 4 sequencing reaction with 5μl
Primers
- Universal primers are supplied by the unit
- Specific primers must be provided by customers | 1.5μM | 5μl per reaction
- Primers for sequencing of BACs or YACs must be provided by customers | 10μM | 5μl per reaction
Sequencing Service List
The platform can sequence most DNA types: plasmid, cosmids, phages and PCR products using universal or specific primers. Contact IBIS for more special enquiries.
Sequencing turn-around time
- 3 ~ 5 working days
International orders
- Yes
Other DNA sequencing related services offered
- De novo sequencing of low complexity genomes (MiSeq)
- Amplicons : MLST and métagénomic (MiSeq)
- Genome resequencing
- Transcriptomes
- Variant calling
- Targeted resequencing
- Genotyping by sequencing
- Sequence Capture
Contact Information
Address: Plate-forme d’analyses génomiques – Pavillon Charles-Eugène-Marchand
1030
Rue de la Médecine – local 0147 (Réception des marchandises)
Université Laval
Québec (Québec) G1V 0A6
CANADA
Contact: Dr. Brian Boyle
Email: brian.boyle@ibis.ulaval.ca
Website: http://www.ibis.ulaval.ca/?pg=sequencage_sanger
Comments
The read length is guarantied over 800-1000bp with a 99% precision (Q20). The could do a lot better if they used the best technology on the market.
Return to the main DNA sequencing service reviews page