General Information
DNA sequencing is one of the core technologies that generates raw data for the development of molecular biology, genomics and bioinformatics fields. DNA sequencing technology is additionally used to study most of the recent DNA markers, which leads to applications in genetics, evolution, ecology and diagnostics. A DNA Sequencing laboratory (DSL) was set up in 1997 to cater to the core DNA sequencing needs of the Department of Biological Sciences. This facility also serves as a valuable training resource for undergraduate students and researchers. There are currently two 16-capillary sequencers and one high throughput 96-capillary sequencer in DSL. Together, these three sequencers enable high levels of productivity and efficiency, offering tremendous potential for de novo sequencing, re-sequencing, microsatellite-based fragment analysis and SNP genotyping projects. The DNA sequencers are handled directly by the DSL users, which makes this facility unique.
DNA Sequencing Instruments Used
1 of ABI3730XL
2 of ABI3130XL
DNA Sequencing Prices
Sequencing is performed by customer:
ABI3130XL
- flat rate of SGD$27.20/ slot (16 samples/ slot)
ABI3730XL
- <48 samples | no service
- 48 samples | SGD$81.60
- 49-64 samples | SGD$108.80
- 65-80 samples | SGD$136
- 80-96 samples | SGD$163.20
Sequencing is performed by staff:
Flat rate of SGD$2.30/ sample
DNA Template Requirements
Not mentioned – Please contact to staff
Sequencing Service List
- Sequencing is performed by customer : first-come-first-serves basic
- Sequencing is performed by DSL staff: run on each Wednesday – samples must be clearly labelled & presented as dry pellet in 1.5ml microfuge tubes and no sample will be stored after sequencing. Please check the rule on the website before ordering.
Sequencing turn-around time
For sequencing is performed by DSL staff: all samples will be processed on the day of submission
International orders
Not mentioned
Other DNA sequencing related services offered
- Next Generation Sequencing
- Genotyping
Contact Information
Address: NUS Department of Biological Sciences – Singapore
Blk S1A #03-01 , Tel: 6516 7984
Staff: Tay Bee Ling
Email: dbstaybl@nus.edu.sg
Website: www.dbs.nus.edu.sg/research/dsl.html
Comments
The facility has a online management system so it allows the facility to be used on a 7 day- 24hours basic. However, all customers have to follow the guideline strictly. Please contact staffs for more details.
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