General Information
The Genomics Core Facility at the Pennington Biomedical Research Center provides services to internal and external researchers. The facility provides four options for sequencing service such as 1) Reaction & Electrophoresis, 2) Electrophoresis only, 3) High-Throughput sequencing, and 4) Fragment analysis.
DNA Sequencing Instruments Used
- Applied Biosystems 3130xl
- Life Technologies SOLiD system 5500xl
DNA Sequencing Prices
Academic
- Electrophoresis only | $3.00
- Fragment Analysis | $3.00
- High-Throughput Sequencing | $3.75
- Reactions and Electrophoresis | $12.50
Non-academic
- Electrophoresis only | $5.00
- Fragment Analysis | $5.00
- High-Throughput Sequencing | $5.00
- Reactions and Electrophoresis | $15.00
DNA Template Requirements
Plasmid
- 25 ng/µL
Single Stranded DNA
- 5 ng/µL
Cosmids, BAC
- 200 ng/µl
PCR Product
- 100-200 bp | 1.5 ng/µL
- 200-500 bp | 5 ng/µL
- 500-1000 bp | 10 ng/µL
- 1000-2000 bp | 20 ng/µL
- >2000 bp | 25 ng/µL
Primers
10 µM
Other information
More than 20 samples must be provided in 96 well plates. Big dye terminator removal service at an additional cost of $2 per sample. Standard primers are provided by the facility.
Sequencing turn-around time
24 hours
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
- De Novo Sequencing
- Targeted Re-sequencing
- Whole Genome Re-sequencing
- Fragment Analysis
- Genotyping
- Micro Array Analysis
- Bioinformatics
Contact Information
Genomics Core Facility
Pennington Biomedical Research Center
6400 Perkins Road
Baton Rouge, LA 70808
USA
Phone: Not listed
Email: gcf@pbrc.edu
Website: http://gcf.pbrc.edu/GCFMain.aspx
Comments
The facility provides wide ranges of sequencing as well as other related services. Sequence will be re-run at no additional charge in case of equipment, reagent or operator error causing sequence failure. Individual and small group training as well as consultations is offered for sequence analysis.
Return to the main DNA sequencing services review page.