General information
The DNA sequencing core facility at Stony Brook State University of New York. They supply DNA sequencing service to over 195 laboratories, both at Stony Brook University, as well as to outside organizations.
Sequencing instruments used
Applied Biosystems 3730
Prices
Single sample (full service)
SUNY Stony Brook Users | $6.46 per reaction
Non-SUNY Stony Brook Users | $6.99 per reaction
Electrophoresis only
SUNY Stony Brook Users | $4.01 per reaction
Non-SUNY Stony Brook Users | $4.33 per reaction
Other
The ABI BigDye reaction mix can be purchased at a discounted price. The cost for SUNY Stony Brook users is $679.00 for a 100 reactions (800 µl) and $734 for external users.
Sample requirements
PCR product
100-200 bp | 1-3 ng
200-500 bp | 3-10 ng
500-1000 bp | 5-20 ng
1000-2000 bp | 10-40 ng
>2000 bp | 40-100 ng
Single-stranded
50-100 ng
Plasmid DNA
250-500 ng
Cosmid, BAC
0.5-1.0 µg
Bacterial genomic DNA
2-3 µg
Primers
3.2 pmol
Sample turn around time
24 hours. All samples collected by 5:00 pm will be posted by 2:00 pm the next day.
International orders
Unknown
GLP DNA sequencing
No
Other DNA sequencing related services offered
- Real time PCR
- Oligonucleotide synthesis
Contact Information
Stony Brook DNA Facility
BST T-6, Rm. 124
Stony Brook, New York 11794-86611
USA
ph. (631) 444-6849
fax. (631) 444-3432
email. dna@osa.sunysb.edu
Comments
Excellent single reaction prices. Stony Brook also offer the BigDye reagent at an excellent price – certainly much cheaper than we can buy it from ABI here in Australia!
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