General Information
The Centre of Plant Structural and Functional Genomics has been established to study the structure and function of nuclear genome in higher plants. The laboratory is a part of the Institute of Experimental Botany and the Centre of the Region Haná for Biotechnological and Agricultural Research. The main focus is on economically important crops like cereals, legumes, bananas and plantains. Their service includes Sequencing, Musa genotyping centre and Flow cytometry
DNA Sequencing Instruments Used
ABI3730XL
DNA Sequencing Prices
DNA sequencing and basic sequence analysis | 200 CZK (excluding VAT)
Capillary electrophoresis | 30 CZK (excluding VAT)
DNA Template Requirements
Plasmids
20 ~ 40 ng/ µl | 15 µl minimum
PCR products
200 ~ 1000bp | 1 ~ 4 ng/ µl | 15 µl minimum
1000 ~ 2000bp | 2 ~ 8 ng/ µl | 15 µl minimu
2000bp | 8 ~ 20 ng/ µl | 15 µl minimum
BAC
100 ~ 200 ng/ µl | 15 µl minimum
Primers
Final primer concentration : 2µM
Other information
Sequencing turn-around time
DNA sequencing and basic sequence analysis | 5~10 working days
Capillary electrophoresis | 2~3 working days
International orders
No information
GLP DNA sequencing
No information
Other DNA sequencing related services offered
No information
Contact Information
Institute of Experimental Botany AS CR
Centre of Plan Structural and Functional Genomics
Slechtitelu 31/893E
783 71 Olomouc
Czech Republic
Website: https://olomouc.ueb.cas.cz/en/
Contact person:
Ing. Marie Seifertová – seifertovam@ueb.cas.cz – Tel: +420 585 238 729
Mgr. Miroslav Valárik, Ph.D. – valarik@ueb.cas.cz – Tel: +420 585 238 714
Comments
The facility only accepts samples prepared in BigDye 3.1 chemistry for analysis. In the case of small orders (less than 24 samples), the delivery time may be longer than normal.
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