General Information
The Centre for Applied Genomics (TCAG) is dedicated to conducting and promoting research in genomics including service and training support for academic, government, and private sector scientists worldwide.
DNA Sequencing Instruments Used
- Applied Biosystems 3730xl
- Illumina HiSeq2500
- Illumina HiScan-SQ
- Ion Torrent Proton
- Roche 454 GS FLX Titanium
- Life Technologies SOLiD 5500xl
DNA Sequencing Prices (CDN)
- $3.50 per successful read
- $12.00 / reaction for BAC, LAMBDA, Cosmid and Fosmid
DNA Template Requirements
-
Plasmids
200-300ng in 7µl
PCR product
- > 4kb | 150-200ng in 7µl
- 2-4 kb | 100-150ng in 7µl
- 1-2 kb | 50-100ng in 7µl
- < 1kb | 50ng in 7µl
- < 500bp | 20ng in 7µl
- < 200bp | 10ng in 7µl
BAC/Cosmid/Phage DNA
- 600-800 ng in 12 µl
Primers
- 5 pmols
Other information
Do not submit Genomic DNA. Failed reads will be charged provided the control from the same sequencing run passes their QC metrics.
Sequencing turn-around time
- 48 hours
International orders
Yes
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
- Next Generation Sequencing
- Library Construction
- Genotyping
- Micro Array Analysis
- Oligo Synthesis
- Bioinformatics
Contact Information
Jo-Anne Herbrick, Facility Manager
The Centre for Applied Genomics
The Hospital for Sick Children
Peter Gilgan Centre for Research and Learning
686 Bay Street, Room 139800
Toronto, Ontario, M5G 0A4,
Canada
Phone: +1 416-813-8140
Fax: +1 416-813-8319
Email: jherbrick@sickkids.ca
Website: http://www.tcag.ca/index.html
Comments
The centre provides wide range of services with state-of-the-art equipment facility. Typical read length for Sanger sequencing is 700 bases. The center currently averages about 3,500 sequence reads per week.