General Information
The mission of the CGI is to provide state-of-the-art expertise in genome technologies like Next Generation Sequencing and microfluidics platforms for genomic analysis while facilitating genomics research for faculty and students across the University of Connecticut campuses.
DNA Sequencing Instruments Used
- ABI 3500
- ABI 3130
- 2 X Illumina NextSeq 500
- 2 X Illumina MiSeq
- NanoPore MinION
DNA Sequencing Prices
- Full Service | $4.00
- Premixed Strip tubes | $3.30
- Premixed 96 well plate | $3.00
DNA Template Requirements
Plasmids
- 100ng per 1KB of plasmid DNA in 6.75ul
PCR products
- 100 – 200bp | 1-3 ng
- 200 – 500bp | 3-10 ng
- 500 – 1000bp | 5-20 ng
- 1000 – 2000bp | 10-40 ng
- >2000 bp | 40-100 ng
Primers
- 3.2μM per 1μL sequencing reaction
Other information
The CGI offers training and a variety of fee-for-service NextGen sequencing and genotyping services for all UCONN affiliated faculty and lab personnel and students.
Sequencing turn-around time
- Not Listed
International orders
- Not Listed
GLP DNA sequencing
- Not Listed
Other DNA sequencing related services offered
The CGI offers multiple variations on sample submission so users have the ability to cut costs by doing part or all of the sequencing reactions and preparing and loading the sequencing run.
Contact Information
Center for Genome Innovation
Beach Hall, Rm. 201
354 Mansfield Road Unit 1131
Storrs, CT 06269
website: http://cgi.uconn.edu/
Comments
The CGI website contains plenty of information to simplify submission of Sanger sequencing samples, they also have submission DropBox fridges at convenient locations around campus.
Return to the main DNA sequencing services review page.