General Information
The Genomics Core Laboratory of the Heflin Center for Genomic Science offers fee based DNA sequencing services for University of Alabama (UAB) members and external clients.
DNA Sequencing Instruments Used
- Applied Biosystems 3730
- Illumina MiSeq
DNA Sequencing Prices
For UAB members
- Individual sequencing reaction | $6/reaction
- Complete 96-well plate | $408/plate
- Quantitation/Dilution/Resuspension | $2/reaction
DNA Template Requirements
Plasmid
- 100 ng/µL | (5-10 µL)
BAC
- 1 µg/uL | (5-10 µL)
PCR Products
- 100-500 bp | 6 ng/µL | (5-10 µL)
- 500-1000 bp | 20 ng/µL | (5-10 µL)
- > 1000 bp | 30 ng/µL | (5-10 µL)
siRNA
- 200 ng/uL | (5-10 µL)
Single Strand DNA
- 100 ng/uL | (5-10 µL)
Primers
Concentration required not mentioned
Other information
The facility provides some free standard primers for sequencing. All samples and primers must be dissolved in distilled water.
Sequencing turn-around time
48-72 hours
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
- Next Generation Sequencing
- Genotyping
- Quantitative PCR
- Sequencing Data Analysis
Contact Information
Heflin Center for Genomic Sciences
1530 3rd Ave. S.
Kaul 412
Birmingham, AL 35294-0024
USA
Phone: +1 205 934 7340
Email: genomics@uab.edu
Website: http://www.uab.edu/hcgs/genomics-core-lab
Comments
The Genomics Core Laboratory provides wide range of genomic services. The facility provides both high and low throughput sequencing services. The genotyping services include whole genome linkage studies, targeted and whole genome gene expression and genome assays. Next Generation Sequencing service is offered in association with the Stem Cell Institute. In addition to standard sequencing analysis, Factura analysis is also available.