General Information
The University of Colorado DNA Sequencing & Analysis Core Laboratory, now a service component of the Department of Pathology, provides DNA sequencing service to its customers in the Colorado Front Range.
DNA Sequencing Instruments Used
- Applied Biosystems 3730
DNA Sequencing Prices
- Premium | $ 8.41 per reaction
- Pre-mix | $ 6.13 per reaction
- Pre-mix (more than 47)| $ 5.42 per reaction
- High throughput | $ 3.40 per reaction
DNA Template Requirements
Plasmids
- 100-200 ng/µL
BAC, Cosmid, & Phage
- 500 ng/µL
PCR products
- 10-15 ng/µL per kb
Primers
5 µM
Other information
Standard sequencing primers are available through the facility.
Sequencing turn-around time
24 hours
International orders
No information
GLP DNA sequencing
No
Other DNA sequencing related services offered
- Fragment Analysis
- Genotyping
- Mutation Screening
- Cell Line Authentication
Contact Information
Christopher Korch, Director
DNA Sequencing & Analysis Core
University of Colorado Anschutz Medical Campus
RC-1 South, Room L18-8400
12801 East 17th Avenue
Aurora, CO 80045
USA
Phone: +1 (303) 724-3167 | +1 (303) 724-3164
Fax: +1 (303) 724-3889
Email: DNA.Core@ucdenver.edu
Website: http://dnasequencingcore.ucdenver.edu/index.html
Comments
This facility is strongly suggested for those doing sequencing on regular basis since their high through-put sequencing service is very cheap at $3.40 per reaction, which requires annual commitment. The core facility has 17 years of experience and offers free consulting on sequencing.
Discontinued Sanger DNA sequencing services.