General Information
The Genomics Facility is one of seven highly specialized laboratories in the University of Guelph’s Advanced Analysis Centre. The Genomics Facility is designated as an Ontario Genomics Platform Affiliate, OGPA, service facility that offers services for DNA sequencing, genotyping, microarray and gene expression analyses.
DNA Sequencing Instruments Used
- Applied Biosystems 3730
DNA Sequencing Prices
- Single reaction | $8 or $9 for external
- Dye Incorporated (DI) | $5
- 48 well plate (DI) | $75
- 96 well plate (DI) | $150
DNA Template Requirements
dsDNA
- 1-5 kb | 50 ng/µl | 25-125 ng
- 5-10 kb | 100 ng/µl| 125-250 ng
- 10-40 kb | 500 ng/µl | 1000 ng
- >40 kb | 1000 ng/µl | 2000 ng
PCR products
- 100-500 bp | 1-10 ng/µl | 10-40 ng
- 500-1000 bp | 20-50 ng/µl | 40-90 ng
- 1000-5000 bp | 50-100 ng/µl | 90-500 ng
Primers
- 5 pmol/µl
Other information
The facility provides sequencing primers for routine vectors. BigDye is available for $100 per 100µl.
Sequencing turn-around time
24 – 48 hours
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
- Fragment Analysis
- Quantitative PCR
- Genotyping
- Micro Array Analysis
Contact Information
Genomics Facility, Advanced Analysis Centre
Room 1401, Science Complex
University of Guelph
488 Gordon St.
Guelph, ON N1G 2W1
Canada
Phone: +1 519 824 4120
Email: genomics@uoguelph.ca
Website: http://www.uoguelph.ca/~genomics/Genomics%20Facility.htm
Comments
Their prices are low and same for the University of Guelph as well as other private sector institutions. The Genomic facility is proceeding with a grant application for Next Generation sequencing (NGS) equipment. The full plate prices are very good.
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