General Information
The High-Throughput Sequencing and Genotyping Unit is part of the Roy J. Carver Biotechnology Center (CBC) at University of Illinois at Urbana. The primary mission of the unit is to facilitate the unraveling of genetic information across the collection of animals, plants and microbes.
DNA Sequencing Instruments Used
- Applied Biosystems 3730xl
- Illumina HiSeq2500
- Illumina MiSeqV3
- Roche/454 GSFLX
DNA Sequencing Prices
Fragment Analysis
- $2.50 per sample
- $93.00 per 96-well plate
DNA Sequencing
- $4.80 per sample
- $355.00 per 96-well plate
Ready-to-Load
- $79.00 per 96-well plate
DNA Template Requirements
PCR Products
- 100-200bp | 10-20 ng /µL
- 200-500bp | 20-30 ng/µL
- 500bp-1kb | 30-50 ng/µL
- 1KB+ | 50-60 ng/µL
Plasmids
- 100-200 ng/µL
Cosmids, fosmids and BACs
- 250-300 ng/µL
Primers
5.0-10.0 pmols/µL
Other information
The Core Facility stocks 20 standard primers that can be used to sequence a variety of templates.
Sequencing turn-around time
24- 48 hours
International orders
Yes
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
- Next Generation Sequencing
- Library construction
- Fragment analysis
- Primer Walking
- Genotyping
- Microarray analysis
Contact Information
High-Throughput Sequencing and Genotyping Unit
Director: Alvaro Hernandez Ph. D.
340 Edward R. Madigan Laboratory
1201 W. Gregory Drive
Urbana, IL 61801
USA
Phone: +1 217 244 3480
FAX: +1 217 265 5066
Email: aghernan@illinois.edu
Website: http://www.biotech.uiuc.edu/htdna
Comments
Facility users other than state of Illinois institutions are subject to a 25.4% indirect cost. For off-campus projects a contract must be negotiated through University of Illinois Grants and Contracts. All work performed by the Roy J. Carver Biotechnology Center (CBC) should be acknowledged in scholarly publications, posters and presentations.
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