General Information
The DNA Sequencing facility is administered through the department of Genetics, Cell Biology and Anatomy of University of Nebraska Medical Center (UNMC). The facility provides both traditional Sanger sequencing as well as Next Generation DNA sequencing services.
DNA Sequencing Instruments Used
- Applied Biosystems 3730
- Illumina HiSeq2000
DNA Sequencing Prices
UNMC
- $6.50 per reaction
Other academic
- $7.80 per reaction
DNA Template Requirements
Single Stranded DNA
- 100-200ng/20µl
Double Stranded (plasmid)
- 600-1200ng/20µl
Cosmid, BAC
- 2-4µg/20µl
Bacterial Genomic DNA
- 8-12µg/20µl
PCR products
- 100-200 bp | 4-12 ng/20µl
- 200-500 bp |12-40 ng/20µl
- 500-1000 bp |20-80 ng/20µl
- 1000-2000 bp |40-160 ng/20µl
- >2000 bp | 80-200 ng/20µl
Primers
- 3.2 pmols/µl
Other information
The core facility provides commonly used primers at no charge. High-Throughput DNA Sequencing and Genotyping Core Facility is funded by Nebraska Research Initiative (NRI).
Sequencing turn-around time
24 hours
International orders
No
GLP DNA sequencing
No
Other DNA sequencing related services offered
- Next Generation Sequencing
- Library Preparation
- Fragment Analysis
- Genotyping
- Micro Array Analysis
Contact Information
James Eudy, Ph.D., Director
985915 Nebraska Medical Center
1010 Durham Research Center II
Omaha, NE 68198-5915
USA
Phone: +1 402-559-3940
Email: jdeudy@unmc.edu
Website: http://www.unmc.edu/genetics/tradseq.htm
Comments
The facility provides services only for UNMC and the academic community in the state of Nebraska. Other facilities include Microarray cores, Mouse Genome and Engineering core, and Electron and Virtual Microscopy cores.
Return to the main DNA sequencing services review page.