General Information
The DNA Core Facility of the University of Texas at Austin is located in Molecular Biology building, which supports the cellular and molecular biology research. The core welcomes user contributions, particularly for tips and tricks with software packages, analysis methods and lab methods.
DNA Sequencing Instruments Used
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Applied Biosystems 3730xl
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Illumina HiSeq
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Roche 454
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ABI SOLiD & Life Tech 5500XL
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PyroMark Q24
DNA Sequencing Prices
University of Texas Investigators
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$3.60 / sample
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$250 / 96well plate
Outside clients
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$6.00/ sample
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$404 / 96 well plate
DNA Template Requirements
Plasmids
- 15 to 30 ng/µl
PCR products
- 2 -5 ng/100 bp
Primers
10 pmol
Other information
Some common primers are available free of charge. Send individual samples in 1.5 ml tubes with 12 ul total volume.
Sequencing turn-around time
24-48 hours
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
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Next Generation Sequencing
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Library Construction
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Pyrosequencing
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Genotyping
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Fragment Analysis
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Quantitative PCR
Contact Information
DNA Core Facility
MBB 1.426
University of Texas at Austin
Cellular and Molecular Biology
2500 Speedway, Stop A4800
Austin, TX 78712
USA
Phone: +1 512 475 7844
Email: dsfcore@austin.utexas.edu
Website: http://www.icmb.utexas.edu/core/DNA/
Comments
The core provides wide range of services. Samples are sequenced with reads over 800 base pairs and a success rate of over 90%. The facility offers “per-read” pricing, which means that customers are not required to purchase a full lane of sequence for NGS projects.