General Information
The Biomolecular Research Facility of University of Virginia provides a range of services for analyzing DNA, peptides and proteins on a fee for service basis. The facility provides expertise for experimental design, sample preparation, protocol optimization and data analysis.
DNA Sequencing Instruments Used
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Applied Biosystems 3730
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Illumina MiSeq
DNA Sequencing Prices
University of Virginia investigators
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1-16 reactions | $7.50/ reaction
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17-48 reactions | $6.89/ reaction
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48+ reactions | $6.63 / reaction
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Full plate sequencing (96 reactions) | $487
External clients
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1-16 reactions | $11.55/ reaction
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17-48 reactions | $10.61/ reaction
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48+ reactions | $10.21/ reaction
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Full plate sequencing (96 reactions)| $749.98
DNA Template Requirements
Plasmids
- 500-1000 ng
Single stranded DNA
- 400-800 ng
PCR product
- 20 ng per 100 bp
BAC DNA
- 1.5-2 µg
Primers
- 8-12 pmole
Other information
Mix the template and primer at the required amount and bring the volume to 14 µl. If submitting without primer, provide the template at the required amount and bring the final volume to 10 µL.
Sequencing turn-around time
24-36 hours
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
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Next Generation Sequencing
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Library Preparation
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Quantitative PCR
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Oligo Synthesis
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Affymetrix GeneChip Expression Analysis
Contact Information
Biomolecular Research Facility,
Jordan Hall loading dock,
University of Virginia Health System,
1340 Jefferson Park Avenue
Charlottesville, VA 22908
USA
Phone: +1 434 924 2356
Fax: +1 434 982 2514
Email: yb8d@virginia.edu
Website: http://www.medicine.virginia.edu/research/cores/biomolec/home-page
Comments
The facility offers a trial period for new users. Detailed procedures of DNA template preparation are available upon request. Typical results yield 750-800 bp sequence with an accuracy of 98% or higher. The data is available for the clients based on their preference from the crude, color electrophoretogram, to the edited sequence.