General Information
The DNA Sequencing and Gene analysis Center provides University of Washington investigators access to high-quality automated DNA Sequencing technology on a cost-sharing basis.
DNA Sequencing Instruments Used
- Applied Biosystems ( model not mentioned)
DNA Sequencing Prices
Not stated
DNA Template Requirements
Single-Stranded DNA
125-250 ng
Double-Stranded/Plasmid DNA
250-500 ng
PCR product
90-150 ng
Primers
3.2 pmoles
Other information
The Center accepts pure plasmids, mini-prep plasmids, M13 clones, cosmids, lambda clones, PCR products, gel isolated fragments.
Sequencing turn-around time
Not mentioned
International orders
No
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
- Fragment Analysis
- Genotyping
- Quantitative PCR
Contact Information
Dr. Kelly, Manager
DNA Sequencing and Gene Analysis Center
University of Washington
H260 Health Science Building
Seattle WA 98195
USA
Phone: +1 (206) 685-4641
Email: edkelly@u.washington.edu
Website:
Comments
The service is available only for University of Washington investigators. Sequencing run typically produces greater than 600 nucleotides of high-quality sequence data. In addition to automated DNA sequencing, the Center specializes in genotype analysis of drug metabolizing enzymes.
Have discontinued Sanger DNA Sequencing services.