General Information
The DNA Sequencing Facility is part of the University of Wisconsin Biotechnology Center. Both Sanger sequencing and next generation sequencing platforms are available, providing inexpensive sequencing of isolated fragments and massively parallel sequencing of random fragments.
DNA Sequencing Instruments Used
- Applied Biosystems 3730xl
- Illumina HiSeq 2500 & 2000
- Illumina MiSeq
- Roche GS FLX+ (454)
- Ion Torrent PGM
DNA Sequencing Prices
- Less than 16 | $10 per sample
- 1-16 | $7.50 per sample
- 17-62 | $5.00 per sample
- 63-96 | $350
- 97-192 | $3.60 per sample
Full Plate Sequencing
- 1 plate | $350 per Plate
- 2+ plate | $300 per Plate
DNA Template Requirements
Plasmids
- <20 kb | 100-200 ng/µl
PCR products
- 10ng/100 bp
Primers
- 5-10 pmol/µl
Other information
Common vector primers are provided. Clients need to provide 24 ul of template, primer, and water in individual 0.2 ml PCR tubes.
Sequencing turn-around time
24 hours
International orders
No information
GLP DNA sequencing
No
Other DNA sequencing related services offered
- Next Generation Sequencing
- Library Preparation
- Quantitative PCR
- Fragment Analysis
- Genotyping
- Microarray Analysis
Contact Information
UWBC DNA Sequencing Facility
Room 2360
Genetics/Biotech Bldg
425 Henry Mall
Madison, WI 53706
USA
Phone: +1 608 263 9880 | +1 608 263 9882
Fax: +1 608 265 6573
Email: DNAseq@biotech.wisc.edu
Website: http://www.biotech.wisc.edu/services/dnaseq
Comments
The facility is equipped with state-of-the-art instruments and handles 10,000-15,000 samples/week. Clients may choose from different levels of service depending on their needs. The UWBC DNA Sequencing Facility can work with researchers to carry out services that may not be listed.
Return to the main DNA sequencing services review page.