General Information
Functional Genomics, Proteomics and Metabolomics Facility, established in 2000 are based in the School of Biosciences at the University of Birmingham and provide scientific services to both the University and external collaborators.
DNA Sequencing Instruments Used
- Applied Biosystems 3730
DNA Sequencing Prices
- Full sequencing service | £5.00 per sample
- Ready to run | £1.00 per sample
DNA Template Requirements
Single Stranded DNA
- 50-100ng
Double Stranded DNA
- 200-500ng
Bacterial genomic DNA
- 2-3µg
PCR products
- 100-200bp | 1-3ng
- 200-500bp | 3-10ng
- 500-1000bp | 5-10ng
- 1000-2000bp | 10-40ng
- more than 2000bp | 40-100ng
Primers
- 3.2 pmol
Other information
DNA and Primer mix together at the correct concentration to a volume of 10ul.
Sequencing turn-around time
Not stated
International orders
No information
GLP DNA sequencing
Not mentioned
Other DNA sequencing related services offered
- Quantitative PCR
- Genotyping
- Micro Array Analysis
- Proteomics Services
Contact Information
Antony Jones, Manager
School of Biosciences
University of Birmingham
Edgbaston
Birmingham
B15 2TT
UK
Phone: +44 (0)121 414 2726
Fax: +44 (0)121 414 5925
Email: a.c.jones.bcs@bham.ac.uk
Website: http://www.birmingham.ac.uk/facilities/genomics/about/sequencing.aspx
Comments
The facility provides competitive prices for external institutions and commercial companies. Generally produce read lengths up to 1000bp. Ready to run service is for researchers wishing to carry out own sequencing reactions using the facility instrument.