General Information
The Institute of Molecular Medicine was established in the Clinical School of the University of Oxford in 1989 to foster research in the field of molecular and cell biology. Sanger DNA Sequencing facility is in-house service for researchers within the Weatherall Institute of Molecular Medicine and John Radcliffe Hospital.
DNA Sequencing Instruments Used
- Applied Biosystems 3730
DNA Sequencing Prices
- Individual reactions | £ 4
- Run only (up to 96 reactions) | £ 50
DNA Template Requirements
Plasmids
- 150 and 300ng/µl
PCR products
- 3-40 ng/µl
Primers
- 3.2pmol/µl
Other information
Many sequencing primers stock is maintained and provided by the facility.
Sequencing turn-around time
No information
International orders
No information
GLP DNA sequencing
Not mentioned
Other cores of WIMM
- Illumina MiSeq Facility
- Flow Cytometry
- HLA Typing
- Bioinformatics
Contact Information
The Weatherall Institute of Molecular Medicine
University of Oxford
John Radcliffe Hospital
Headington
Oxford OX3 9DS
UK
Phone: 01865 222623 | +44 1865 222443
Fax: +44 1865 222737
Email: reception@imm.ox.ac.uk
Website: http://www.imm.ox.ac.uk/sanger-dna-sequencing
Comments
Typical read length is 650-900 bases with PHRED scores of 20. Other core facilities at WIMM provide extensive access to technology, skills and knowledge.
Return to the main DNA sequencing services review page