General information
The University of Maine DNA sequencing core facility provides DNA sequencing and genotyping services for university and outside clients.
Sequencing instruments used
Applied Biosystems 3730
Prices
Single sample
Full service | $12.00
Unedited | $10.00
Ready-to-run (electrophoresis only) | $4.00
Volume discounts
The University of Maine DNA sequencing core facility offers the following per month discounts:
| 50-99 reactions | 5% discount |
| 100-149 reactions | 10% discount |
| 150-199 reactions | 15% discount |
| 200 or more reactions | 20% discount |
Sample requirements
Plasmids
100 ng/µl | 5 µl minimum
ssDNA
10 ng/µl | 5 µl minimum
PCR products
5 – 10 ng/µl | 10 µl minimum
BAC
200 ng/µl | 5 µl minimum (1 µg total)
Bacterial genomic DNA
500 ng/µl | 5 µl minimum
Primers
5 pmol/µl
Common universal primers are provided free.
Other
DNA template and primer should be supplied in 10 mM Tris-HCl, pH 8.0 or in water.
Sample turn around time
Two days
International orders
Yes
GLP DNA sequencing
No
Other DNA sequencing related services offered
- GeneScan fragment analysis
- Primer walking sequencing
- Full service sequencing
- Electrophoresis only sequencing
- Fragment analysis of AFLP and microsatellites
Contact Information
The University of Maine DNA Sequencing Facility
5735 Hitchner Hall, Room 366
Orono, ME 04469-5735
USA
ph. 207 581 2795
fax. 207 581 4867
email. dnaseq@maine.edu
Comments
The University of Maine DNA Sequencing Facility offer excellent single reaction prices. The monthly volume discounts are nice, especially if you wish to run a lot of single reaction samples.
Return to the main DNA sequencing services review page.