General information
University of Missouri DNA Sequencing Core provide DNA and other related sequencing services. The facility also has an two Illumina HiSeq 2500 and two MiSeq nextgen instruments.
Sequencing instruments used
- ABI 3730xl
- Illumina HiSeq
- Illumina MiSeq
Prices
Single Sample
- Missouri customers | $5.50
- Non-Missouri customers | >$5.50 (contact for quote)
- Non-Academic customers | >$5.50 (contact for quote)
96-Well Trays
- Missouri customers | $408.00 ($4.25 per sample)
- Non-Missouri customers | >$408.00 (contact for quote)
High Throughput DNA Sequencing
- >200 96 well trays | $2.75 (contact for quote)
Sample Requirements
Single Tube
PCR product
- 50 – 275ng | 16µL (with primer)
Plasmid ssDNA
- 275 – 550ng | 16µL (with primer)
Plasmid dsDNA
- 1000 – 2000ng | 16µL (with primer)
BACs, Cosmids and Fosmids
- 2500 – 4000ng | 16µL (with primer)
Primers
Templates should contain 15 – 26 pmol of primer.
Other
All non-tray samples should be submitted in 1.5 mL tubes with a total volume of 16 µL. Tubes should be labelled with on the lid with the last three digits of the LIMS code.
96- well Tray
PCR product
- 10 – 50ng | 6µL (with primer)
Plasmid ssDNA
- 50 – 100ng | 6µL (with primer)
Plasmid dsDNA
- 200 – 500ng | 6µL (with primer)
BACs, Cosmids and Fosmids
- 500 – 1000ng | 6µL (with primer)
Primers
Templates should contain 5 – 10 pmol of primer.
Other
The trays should be obtained from the core facility.
Sample turn around time
24 − 36 hours
International orders
Unknown
GLP DNA sequencing
Unknown
Other DNA sequencing related services offered
- SNP detection
- Fragment analysis
- Genescan
- Illumina Genotyping service
- Illumina Library Construction
- DNA/RNA QC
- Metagenomics & 16S rDNA sequencing
Contact Information
DNA Core Facility University of Missouri – Columbia
216 Bond Life Sciences Center
1201 Rollins Street
Columbia, MO 65211-7310
USA
ph. (573) 882-0428
fax. (573) 884-5552
email. dna-core@missouri.edu
Comments
The University of Missouri DNA Sequencing Core has a good DNA troubleshooting guide. A well organized site.
Return to the main DNA sequencing services review page.