General Information
The DNA Analysis Facility on Science Hill at Yale was founded in 2003 to offer DNA core services to Yale researchers and outside researchers in industry and academia. Based at Yale University in New Haven, Connecticut.
DNA Sequencing Instruments Used
ABI 3730xl (96 capillary instrument)
DNA Sequencing Prices
Full service DNA sequencing (pre-mixed template and primer)
Single tube | $4.75
Full plate | $240.00
DNA Template Requirements
Plasmids
Sample must be supplied premixed with primer
500ng template + 3 – 5 pmol of primer in 12 µl final volume
PCR products
10-20 ng per 200 bases + 3 – 5 pmol of primer in 12 µl final volume
Primers
Some universal primers are available at no charge.
Other information
Offers, run-ready (with or without purification of samples) and Full Service Sequencing of PCR Products. Add on services including alternative chemistries and enhancers for difficult templates. Does not accept credit cards. Will bill outside institutions against a pre-approved Purchase Order. Traces are retrieved electronically via the secure Finch Suite Laboratory Information Management System by Geospiza.
Sequencing Turn Around Times
48 hours
International orders
Yes
GLP DNA sequencing
No
Other DNA sequencing related services offered
Fragment Analysis of AFLP and Microsatellites
- $1.00 per sample. Choice of a number of different Rox or Liz size standard.
- $0.75 per sample if you supply your own size standard.
- No minimum submission size.
Human cell line authentication
- Promega GenePrint10 system | $10.00
The following dyes sets are supported:
- DS-31 5 Dye Set – VIC [green] , NED [yellow], 6-FAM [blue], PET [red] & LIZ size standard [orange].
- DS-30 4 Dye Set – HEX [green], NED [yellow], 6-FAM [blue], ROX size standard [red].
Contact Information
FedEx, DHL or UPS
DNA Analysis Facility on Science Hill
Attn: Carol Mariani
170 Whitney Ave.
Esc Room 150
New Haven, CT 06511
USA
United States Postal Service
DNA Analysis Facility On Science Hill
Attn: Carol Mariani
PO Box 208105
New Haven, CT 06520
USA
website. https://dna-analysis.yale.edu/
email. dnanalysis@yale.edu
phone. 1-203-432-7394
Comments
The Yale DNA Analysis Facility on Science Hill offer a high quality, yet very cheap service. The sequencing quality is outstanding and offer PeakTrace and LongTrace processing. Yale have recently used PeakTrace to generate the world record (to my knowledge) for longest read – over 1307 Q20+ bases from a single read! They are also very friendly and professional to deal with. Highly recommended.
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