General Information
The Core Genomic Facility was established in 1994 to provide a DNA sequencing service to University of Sheffield researchers. Today they also serve UK and European academic institutes as well as to private sector companies.
DNA Sequencing Instruments Used
ABI 3730
DNA Sequencing Prices
Special rates apply for samples supplied in 96 well plates that are to be sequenced using a single primer
DNA Template Requirements
Plasmids
10ul at a concentration of 100ng/ul
PCR products
10ul at a concentration of 50ng/ul
Primers
10ul at 1 uM
Sequencing turn-around time
Two working days
International orders
The facility also serves to European academic institutes as well as to private sector companies.
GLP DNA sequencing
No information
Other DNA sequencing related services offered
- DNA extraction
- Cell line identification (STR)
- Microsatellite analysis
- DNA/RNA quantification
- Monoclonal antibody sequencing
Contact Information
Core Genomic Facility
FU27/28 Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX
Telephone: +44 (0)114 215 9650
Email: dnahelp@sheffield.ac.uk
Website: http://genetics.group.shef.ac.uk/dna-sequencing.html
Comments
The facility developed specially modified protocols for difficult to sequence samples, for instance, those with high GC content.