General Information
Sistemas Genomicos is established in 1998 and it is the first Spanish firm and one of the first in Europe which specializes in DNA sequencing. Sistemas Genomicos occupies the leading position in DNA and RNA analyses in Spain and has a capability of successfully taking international genome sequencing projects. This commitment is shared among 60 professionals with recognized experience in the Molecular Biology and Genetics field.
DNA Sequencing Instruments Used
- ABI3730XL
- HiSeq 2000
- MiSeq
DNA Sequencing Prices
Not mentioned – contact to staff
DNA Template Requirements
Sample needs to be sent in 1.5µl Eppendorf tube or 96-well plate, cold storage/ dry ice.
Simple strand sequencing
Economic service
- Cloned DNA | 2.5µl | 100ng/µl | and dH20 | 13 µl
- PCR products up to 1200bp | 2.5µl | 10ng/µl | and dH20 | 13 µl
- PCR products up to 1200~3000bp | 2.5µl | 25ng/µl | and dH20 | 13 µl
- PCR products more than 3000bp | 2.5µl | 50ng/µl | and dH20 | 13 µl
- Customer’s specific primer | 3.5µl with the concentration of 1µM
- Common universal primers are provided free.
Standard service (with qualification of sample) or High quality service (with qualification of sample and sequence edition)
- PCR product less than 800bp | minimum concentration of 1.5ng/µl | minimum volume of 15µl
- PCR product less more than 800bp | minimum concentration of 2~10ng/µl | minimum volume of 15µl
- Cloned DNA | minimum concentration of 20ng/µl | minimum volume of 15µl
- Customer’s specific primer | minimum volume of 10µl with the concentration of 1µM
- Common universal primers are provided free.
- Purification and extraction are also supplied with extra fee
Sequencing project (primer walking) – with verification of DNA sample
- Double-stranded sequencing: complete sequencing of both strands of a DNA fragment (4x)
- Diagnostic sequencing : complete sequencing of both strands of a DNA fragment
- Analytical Sequencing: complete sequencing of DNA fragment
Number of reactions = insert size(in nucleotides)/750 x Redundancy
( Redundancy: analytical (1x), double-stranded (2x), diagnostic (4x) )
- Cloned DNA in a plasmid | 200ng/ reaction
- PCR product | 5-10ng/ reaction
- Customer’s specific primer | minimum volume of 10µl with the concentration of 1µM
- Common universal primers are provided free.
Sequencing Service List
- sequencing service
- project service
- extraction service
- purification service
Sequencing turn-around time
- depending on the service, it will take 48 hours or more from arrival time of samples
- sequencing project: from 10 working days/kb insert
International orders
Yes
Other DNA sequencing related services offered
- Massive Sequencing – NGS
- Bioinformatics
Contact Information
Spain
Pargue Technologico de Valencia
Ronda G. Marconi, 6 -46980 Paterna (Valencia)
Spain
Tel: +34 961 366 150
Mexico
Avenida Insurgentes Sur 1793, Numero 210
Colonia Guadalupe Inn, Delegacion Alvaro Obregon, C.P. 01020 Distrito Federal
Mexico
Tel: +52 1 55 47317278
Email: info@sistemasgenomics.com
Website: https://www.sistemasgenomicos.com/web_sg/webing/solicitud-servicio.php
Comments
Sistemas Genomicos is specialized in a diagnosis of genetic diseases and Sanger DNA sequencing is used as one of tools for molecular diagnoses. The facility pays high attention on quality policy and gains the ISO9001, ISO117025. Their biomedicine protocols are validated by the FDA and EMEA.
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