interpreting sequencing data

[FH]

Hello fellas!
I wanted to, kind of, confirm that in order to interpret sequencing results of a PCR product whether one should find the complentary strand of the product and than check for mutations? My PCR products match the original sequence at another exon….how should I proceed? HELP ASAP! PLEASEEE!!!

[Daniel]

The simplest way is just to blast your sequence at the NCBI site. If your sequence isn’t in GenBank (unlikely these days) then you can always compare to your known sequence using the blast 2 sequence option.

Daniel Tillett
Nucleics Support

[Author]

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