PCR primer concentration- pls read this n pls reply!!

[DK]

I’m working with DNA aptamers and i cannot figure out how to set up my PCR. My oligos are around 100 bases in length and i need to perform a PCR after each Selex round.. the general PCR setup suggests a primer concentration of 1uM..that wud be around 100pmoles of primer in a 100ul reaction rite??So if i wanted to amplify my oligos..for say 10 rounds.. how do i calculate the amount of template i start with? The way i figure…with a lot of calculation.. is that i cannot even amplify..say 1 pmol of my oligos?? Is there an easier way to calculate the template vs primer ratio? Keeping in mind the size of my oligos?<100 bases>

[Author]

Posts