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sequencing long PCR fragment

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    • July 8, 2009 at 5:55 am #1167
      jelena
      Participant

      Hi,

      we want to sequencing 1600 bp PCR fragment but we only have 50 cm capillary and pop-7. Can we sequencing with this?

    • July 21, 2009 at 5:57 am #1168
      Anonymous
      Inactive

      Hi Jelena,

      The 1600 bp PCR fragment has to be first sequenced using the forward and reverse PCR primers followed by a 3rd primer designed based on either the forward or reverse data and finally the data has to be aligned.

    • July 22, 2009 at 5:58 am #1169
      Daniel
      Keymaster

      Hi Jelena

      You shouldn’t need a internal primer if you run your traces through our free LongTrace or PeakTrace software. This should increase the read length over 1000 bases and will give you enough good sequence to get the entire 1600 bases with only the two end reads.

      Daniel Tillett
      Nucleics Support

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