we want to sequencing 1600 bp PCR fragment but we only have 50 cm capillary and pop-7. Can we sequencing with this?
The 1600 bp PCR fragment has to be first sequenced using the forward and reverse PCR primers followed by a 3rd primer designed based on either the forward or reverse data and finally the data has to be aligned.
You shouldn’t need a internal primer if you run your traces through our free LongTrace or PeakTrace software. This should increase the read length over 1000 bases and will give you enough good sequence to get the entire 1600 bases with only the two end reads.
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