Taq Dilution

[Anonymous]

Hi,

I’d like to ask for instructions about Taq DNA polymerase dilution. To avoid pipetting errors I have to dilute Taq, which is supplied in a concentration of 5 units / microL, to a concentration of 0.5 units / microL . I just wonder if I can use PCR reaction buffer for the dilution process. I know Taq is supplied in a special buffer which contains Glycerol , surfactants , etc . and I would prefer to use a solution of similar composition for dilution but that needs to be purchased and there doesn’t seem to be many suppliers for that anyway. So I wonder if I can make a 1:10 dilution using just the PCR buffer and store in aliquotes safely . I am highly concerned about storing Taq in just PCR buffer. I know I could just make a dilution, use the amount I need , then throw away the rest of the solution but I just can’t afford that. I need to store the rest for future use.

Thank you in advance.

[Daniel]

Yes you can store Taq in PCR buffer for a short time. Don’t add the dNTPs. It will last at least a week in the fridge like this.

Daniel Tillett
Nucleics Support

[Author]

Posts