Here are some new publications that mention the use of PeakTrace in their methods sections. While most people using PeakTrace are unaware they are using it as it is being performed automatically for them by their DNA sequencing service provider, it is always good to see small-scale users making use of the free online version of PeakTrace.
Archives for August 2015
Auto PeakTrace 6 RP Software Released
We have released Auto PeakTrace 6 RP. This is a major update to our PeakTrace RP software and offers a number of improvement and new features as well as the usual improvements and fixes. The full list of changes are.
- New Feature. Clean baseline.
- New Feature. Extra normalization.
- Parallel installation of Auto PeakTrace 6 RP with Auto PeakTrace RP.
- Improved handling of capillaries that run slower than expected.
- Improved signal to noise determination.
- Improved trimming of noise level data from traces.
- Improved detection of failed traces.
- Improved basecalling of traces without KB quality scores.
- Improved basecalling of short traces.
- Improved q average trim.
- Additional error messages to help identify the cause of problems.
- Updated visual design and help manual.
- Bug fixes.
Many of these changes will be automatically applied to users of previous versions of Auto PeakTrace RP as they are controlled by the remote server software, however the two new features (clean baseline and extra normalisation) can only be utilised via the Auto PeakTrace 6 RP. This major update is available from the Nucleics Downloads page and it is highly recommend that all users of PeakTrace RP upgrade to this version.
How to find the Auto PeakTrace 6 License Code
The license code must be supplied to upgrade Auto PeakTrace 5 to Auto PeakTrace 6. This information is contained on the Licensing Information window of Auto PeakTrace 6.
- Open the options window and click on License button.
- Mouse over the License Code and right click to copy.
- Email the License Code to Nucleics (or your local supplier). The code should be a series of numbers and letters seperated with five dashes.
If you are using the Linux version of PeakTrace you will need to install Auto PeakTrace 6 on a Windows computer and then plug in the PeakTrace USB Key as there is currently no way to obtain the license code using the command line version of PeakTrace.
Auto PeakTrace 6 Released
A major update to PeakTrace has been released – PeakTrace 6. This version has a large number of improvements and new features and it is highly recommended that all users update to this version (it is a free upgrade). Both the Windows (Auto PeakTrace) and Linux versions (PeakTrace) are now available from the Nucleics Downloads page. You will need to update your system as described in the How to upgrade to Auto PeakTrace 6.
The full list of changes for PeakTrace 6 include.
- New Feature. Clean baseline.
- New Feature. Extra normalization.
- New Feature. QualTrace III integration.
- New Feature. Trace reports.
- Parallel installation of Auto PeakTrace 6 with Auto PeakTrace 5.
- Improved handling of capillaries that run slower than expected.
- Improved signal to noise determination.
- Improved trimming of noise level data from traces.
- Improved detection of failed traces.
- Improved basecalling of traces without KB quality scores.
- Improved basecalling of short traces.
- Improved q average trim.
- Changed default settings for the command line version of PeakTrace. The default settings are now:
- trace file: .ab1
- .seq file: abi
- fuse trace: 1
- Additional error messages to help identify the cause of problems.
- New licensing system requiring a license update.
- Updated visual design and help manual.
- Bug fixes.
PeakTrace Online Now Using PeakTrace 6
We have updated the free version of PeakTrace Basecaller to use PeakTrace 6. This update is a significant upgrade for PeakTrace and it generates superior trace files compared to the 5.90 version. We will be releasing the standalone versions for Windows and Linux of PeakTrace 6 over the next few days, but in the meantime you can see some of what PeakTrace 6 can do now on your own traces.