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RNA integrity

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    • November 11, 2008 at 5:50 am #1549
      DanielT
      Participant

      How to determine the integrity of the RNA beside by using the commercial kit?

    • November 4, 2010 at 5:50 am #1550
      Anonymous
      Inactive

      Dear Sir/Madam,

      I am a Ph.D student on molecular plant pathology. I have been tryning to extract RNA from banana peel to construct a cDNA library. I have optimized the RNA extraction protocol using literitures.However RNA concentration some what low but it can be visualized through 1% agarose non denaturing gel but it can not be visualized by denaturing gel. Therefore I want to know the reason for this and ways to improvemy RNA yield.

      Kind regards,
      Aruna.

    • November 17, 2010 at 5:51 am #1551
      Daniel
      Keymaster

      You really need to provide more information before anyone can help.

      Daniel Tillett
      Nucleics Support

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